Metodiche innovative nel laboratorio di ricerca clinica

The application of biophotonics, like vibrational spectroscopies and biosensors, offer valuable alternatives to the traditional biomolecular techniques, including ELISA, Real Time-Polymerase Chain Reaction (RT-PCR), mass spectroscopy, for the diagnosis and monitoring treatment of several diseases. Often the conventional methods require consistent sample amount, rely on operator expertise for manual procedure steps, can be time- consuming and provide only partial information about the biochemical features.
Instead Raman Spectroscopy (RS) ensures high chemical specificity and fast analytical procedure due to the minimal sample preparation, due to the described advantages, RS has been successfully applied in the diagnostic field and proposed as potential device for the analysis of liquid biopsy in several pathologies.
We propose in this presentation an overview of our biophotonic platform as promising methods used in the field of extracellular vesicles (EVs)-related biomarker discovery and saliva analysis.
Herein, we propose the Raman Spectroscopy (RS) and Surface Enhanced Raman Spectroscopy as promising and valuable tools for the diagnosis, prognosis and monitoring of the pathological state in neurological, pulmonary and cerebrovascular diseases.